目录简介 | National food safety standard
Food microbiological examination: Listeria monocytogenes
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1 Scope
This standard specifies the testing methods of Listeria monocytogenes in foods.
This standard applies to testing of Listeria monocytogenes in foods.
2 Apparatus and materials
Except for routine sterilization and culture equipments in microorganism laboratories, other equipments
and materials are as follows:
2.1 Refrigerator: 2 - 5.
2.2 Thermostatic incubator: 30±1, 36±1.
2.3 Homogenizer.
2.4 Microscope: 10× - 100×.
2.5 Electronic balance: with a reciprocal sensibility of 0.1g.
2.6 Conical flask: 100mL, 500mL.
2.7 Sterile pipette: 1mL (with 0.01ml graduation), 10ml (with 0.01ml graduation).
2.8 Sterile plate: with a diameter of 90mm.
2.9 Sterile test tube: 16mm×160mm.
2.10 Centrifuge tube: 30mm×100mm.
2.11 Sterile syringe: 1mL.
2.12 Staphylococcus aureus (ATCC25923).
2.13 Rhodococcus equi.
2.14 Mus musculus albus: 16g - 18g.
2.15 Automatic microorganism identification system.
3 Culture medium and reagents
3.1 Tryptone soya broth containing 0.6% yeast extract (TSB - YE): see section A.1 in Appendix A.
3.2 Trypticase soy agar containing 0.6% yeast extract (TSA - YE): see section A.2 in Appendix A.
3.3 Listeria enrichment broth LB (LB1, LB2): see section A.3 in Appendix A.
3.4 1% acriflavine HCl solution: see section A.3.2.1 in Appendix A.
3.5 1% nalidixate sodium (naladixic acid) solution: see section A.3.2.1 in Appendix A.
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