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Foreword
1. Standard Plate-count Bacteria
2. Total Coliforms
3. Thermotolerant Coliform Bacteria
4. Escherichia Coli
5. Giardia
6. Cryptosporidium
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1. Standard Plate-count Bacteria
1.1 Plate Counting Method
1.1.1 Scope
This standard provides to determine total number of bacterial colony in drinking water and its source by plate counting method.
This method applies to determination of bacterial colony in drinking water and its source.
1.1.2 Terms and Definitions
The following terms and definitions apply to this standard.
1.1.2.1
Standard plate-count bacteria
Standard plate-count bacteria in 1 mL water sample after 48 hours of cultivation on nutrient agar under aerobic condition of 37℃.
1.1.3 Medium and Reagent
1.1.3.1 Nutrient Agar
1.1.3.1.1 Ingredients:
A peptone 10g
B beef extract 3g
C sodium Chloride 5g
D agar 10g~20g
E distilled water 1000mL
1.1.3.1.2 Method: Mix the ingredients mentioned above and heat them to dissolve, adjust pH to 7.4~7.6, and put them separately into glass container (If the agar contains more impurities, it should be filtered firstly), after 20 minutes of sterilization of 103,43 kPa (121℃,15lb) store them in dark cold place as preparation. |
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