GB 5413.10-2010 National food safety standard Determination of vitamin K1 in foods for infants and young children,milk and milk products(Text of Document is in English)

01/06/2010

12P

$60.00 USD

1. Scope
This present National Standard specifies the method for determination of Vitamin K1 in foods for infants and young children, milk and milk products.
This present National Standard is applicable to the determination of Vitamin K1 in foods for infants and young children, milk and milk products.
2. Normative references
The following standards contain provisions which, through reference in this text, constitute provisions of this present standard. As for the dated references, all the amendments or revisions after them except the corrigenda are not applicable to this present standard. As for the references that are not dated, their most recent editions are applicable to this present national standard.
3. Principle
The fats and unsaturated fatty acids in test sample are degraded by lipase first; then, for the starch of the samples requiring degradation with starch amylase, after saponification by alkali, Vitamin K1 is extracted by normal hexane. After separation by liquid chromatography, post-column reduction of Vitamin K1 is carried out; the fluorescence detector is used for detection, and external standard method is used for quantification.