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1. Scope: This standard is formulated a microbial method for determination of vitamin B12. This standard is applicable to Determination of Vitamin B12 in Foods for Infants and Young Children, Raw Milk and Dairy Products. 2. Principle: Lactobacillus leichmannii is very sensitive to the existence of vitamin B12. So it can be used for determination of vitamin B12 in sample. 3. Reagent, strain and culture medium All reagents, if no special specification, refer to analytic reagent; All experiment water, if no special order, refers to level 3 water.
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