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Contents Foreword 1 Scope 2 Normative References 3 Abbreviations 4 Biosecurity measures 5 The measures for waste disposal and pollution prevention 6 Principle 7 Reagents and materials 8 Main instruments and equipments 9 Methods summary and detection procedures 9.1 Method summary 9.2 Inspection process 10 Operating steps 10.1 Sample preparation, enrichment culture and separation 10.2 Preparation of template DNA 10.3 PCR amplification 10.4 DHPLC detection 10.5 The control settings of quality control 11 Results and determination 11.1 Quality control standard: 11.2 Results determination and report Annex A (Normative) Sequences of primers used in the PCR-DHPLC detection of pathogen in food Annex B (Informative) References List Annex C (Informative) Examples of DHPLC detection map of pathogen in food |